To evaluate the features of carotid web (CW) by ultrasonography.

A total of 66 patients with CW were retrospectively enrolled from January 2018 to June 2019 at Xuanwu Hospital, Capital Medical University. All patients were examined by both ultrasonography and CTA, and were divided into either a<50% stenosis group (54 cases) or a ≥50% stenosis group (12 cases). The ultrasonographic characteristics of CW, including the length, thickness, direction (forward or backward to the flow), acute angle between the web and carotid wall, and thrombus between the web and carotid wall were compared between the two groups. The stenosis degrees of carotid artery were compared between patients with and without ischemic stroke.

Forty-two (42/66, 63.6%) patients were diagnosed with CW by initial CDFI examination, of whom 21 (21/66, 31.8%) were diagnosed with ulcerative plaque and 3 (3/66, 4.5%) were diagnosed with carotid dissection at first but confirmed by second examination. There were no differences in the web length, thickness, direction, or thrombus detected between the two groups (P>0.05). The angle between the web and carotid wall in the<50% stenosis group was significantly smaller than that of the ≥50% stenosis group (median angel 39o vs 73o, P=0.002), and the percentage of patients with an angle≤ 60o in the<50% stenosis group was significantly higher than that of the ≥50% stenosis group (74.1% vs 41.7%, P=0.042). The diameter of the residual carotid artery at CW location in the<50% stenosis group was significantly larger and peak systolic velocity was significantly higher in the<50% stenosis group than in the≥50% stenosis group (P<0.001). The stenosis degrees of carotid artery were not statistically different between patients with and without ischemic stroke (P=0.321).

Ultrasonography can be used to evaluate the characteristics of carotid web in 2D and color mode. When the angle between the CW and carotid wall is large, the carotid artery stenosis ≥ 50% is more likely to happen, but carotid artery stenosis is not the main cause of ischemic stroke.

The aim of the present study was to evaluate the efficiency of sling exercise, therapeuticultrasound, therapeuticultrasound and sling exercise in patients to alleviate pain and improve lumbar function with lumbar disc herniation.

Individuals were selected from a list of patients being followed at the department of Rehabilitation in the third hospital of Hebei Medical University. 30 patients who were diagnosed with lumbar disc herniation were collected, the diagnoses were established upon medical history, physical examination, and results of imaging studies. The patients were randomly divided into three groups: therapeuticultrasound group received 14 sessions of ultrasonic therapy to the lumbar region, Sling exercise group received 14 sessions of sling exercise, and therapeuticultrasound and sling exercise group received therapeuticultrasound and sling exercise therapy 14 sessions of therapeuticultrasound to the lumbar region,7 sessions per week, 2 weeks. The VAS and ODI were compared with the assessment of the patients before and at the end of the therapy.

At the end of treatment, three groups VAS scores (F=3.069, P=0.043) and ODI scores (t=12.676, P<0.001) was lower than that at the beginning of treatment (P<0.05), at the end of treatment the VAS scores (F=59.400, P<0.001) and of the ODI scores (t=12.737, P<0.001) of ultrasonic and sling exercise therapy group was lower than the other group, the difference is significantly.

All three groups could reduce pain and improve lumbar function, and the ultrasonic and sling exercise therapy was most effective for lumbar disc herniation treatment in the three groups.

To analyze the treatment of different types of traumatic cerebral infarction in children, and explore its pathogenesis in combination with literature so as to improve the cure rate and reduce disability rate.

The clinical data of 42 cases of traumatic cerebral infarction in children were retrospectively analyzed in The Hospital of 81^st Group Army PLA from January 2015 to December 2019. The diagnosis of traumatic cerebral infarction in children was made by CT scan and MRI scan. According to different conditions, children with traumatic cerebral infarction were classified, and different treatment strategies were selected. Children with lacunar infarction were treated with calcium antagonists and neurotrophic drugs, supplemented by hyperbaric oxygen and exercise rehabilitation. The children of focal cerebral infarction and complex cerebral infarction treated by junior dehydrant and hormone also included the calcium antagonist and nutritional nerve drugs. The therapeutic regimen perhaps adjusted by the evolution of the disease. The children of extensive cerebral infarction underwent emergency cranial decompression, and was treated by reducing intracranial pressure and preventing complications after operation. The treatment results and recovery were observed.

In 42 cases of traumatic cerebral infarction in children, 35 cases (83.3%) were good recovery, 4 cases (9.5%) were moderate disability, 2 cases (4.8%) were severe disability, 1 case (2.4%) died, and no vegetative state. The good recovery rate of lacunar infarction was 100%, that of focal cerebral infarction was 62.5%, that of mixed cerebral infarction was 60%, and that of extensive cerebral infarction was 50%.

It is of great significance to improve the therapeutic effect and prognosis of children with traumatic cerebral infarction to adopt different treatment schemes for different types of cerebral infarction.

To characterize the patients with supratentorial hypertensive intracerebral hemorrhage (ICH) in the Tibetan Plateau over an altitude of 4000 meters.

A total of 68 cases with supratentorial hypertensive ICH were retrospectively included in Ali Regional People’s Hospital from January 2017 to September 2018. The clinical and laboratory data were collected. A simple linear correlation analysis was applied to analyze the correlation between the amount of bleeding and sex, age, nationality, time from onset , systolic blood pressure (SBP), diastolic blood pressure (DBP), hemoglobin (Hb), serum triglyceride (TG), and cholesterol. According to computed tomography (CT) findings, 68 cases were divided into the basal ganglia ICH group (33 cases) and the lobar ICH group (35 cases). The characteristics between two groups were analyzed using t-test or χ² test.

The data of sex, age, nationality, time from onset, SBP, DBP, Hb, TG, and cholesterol of 68 cases on admission did not show any definitive correlation with the hematoma volumes (P＞0.05). The SBP and DBP of patients in the basal ganglia ICH group were significantly higher than that in the lobar ICH group, respectively [(184.9±28.5) mmHg vs (164.6±24.4) mmHg; (113.0±18.1) mmHg vs (103.0±18.4) mmHg] (t=0.499, 0.486; P=0.002, 0.033).

The relevant factors of hematoma volumes in patients with ICH in plateau area were not yet clear. Diastolic and systolic blood pressure of patients in the basal ganglia ICH group was higher than that in those in lobar ICH group.

To compare the efficacy evaluation of electrospun composite biomaterials and a porcine small intestine submucosa mesh for hernia repair.

A randomized, single-blind, controlled multicenter trial was performed in 3 hospitals in Shanghai. Eligible adult patients with primary unilateral reducible groin hernias were randomly assigned (1∶1) to electrospun composite biomaterials (experimental group) or porcine small intestine submucosa (control group) mesh groups. Patients were treated with the tARB technique and assessed at 1,3 and 6 months after the surgery. The primary endpoint was hernia recurrence. The secondary endpoints were postoperative complications including groin pain and operative site infections.

172 patients were assigned to experimental (n=86) and control (n=86) groups. At 6 months follow-up, postoperative complications occurred in 5 patients (5/86, 5.95%) and 2 (2/86, 2.35%) patients in the control and experimental groups, respectively (P>0.05). There was no significant difference in VAS or SVS score between the two groups.

We demonstrate that electrospun composite biomaterial mesh can be used as a ideal choice for inguinal hernia repair. Electrospun composite biomaterial has the characteristics of low recurrence rate, absorbability and long-term comfort.It can be further applied in clinical practice in the future.

Pancreaticoduodenectomy(PD)remains one of the most complicated hepatobiliary operations. The development of minimally invasive surgery for PD has always been an hot spot. Laparoscopic pancreaticoduodenectomy(LPD) has not been widespread carried out due to its difficulty and long learning curve. LPD accounts for 9% of all PD, according to the National Cancer Data base. Compared with laparoscopic surgery, robotic surgery system has significant advantages in the field of minimally invasive PD, including stereotactic amplified vision, filtering hand tremor and simulating the wrist. The article would review the surgical techniques and notes, which could provide clinical reference for other surgeons.

This study aims to explore potential novel drug resistance mechanism of NCI-H460 cells to the neddylation inhibitor MLN4924.

NCI-H460 cells were treated by MLN4924 to screen and establish drug-resistant cell lines. High throughput transcriptome sequencing was performed on the drug-resistant cell lines, MLN4924 treated 8-hour cell lines, 48-hour cell lines, and control cells. Then we screened differentially expressed genes based on sequencing results and conducted pathway enrichment analysis. The comparison among multiple groups was used by one-way ANOVA, and the pairwise comparison between two groups were performed by Dunnett-t test.

Three MLN4924-resistant cell lines were constructed. Transcriptome sequencing analysis showed that compared to the normal control group, there were 5 303, 4 186, 3 388, 3 675, and 3267 differentially expressed genes for MLN4924 treated 8-hour, 48-hour cell lines, and three drug-resistant cell lines, respectively, including genes such as ABCA9, ABCA13, CYR61, CYP39A1, etc. The RT-qPCR experiment further confirmed the significant high expression of ABCA9 and CYR61 in the transient treated cells and drug-resistant cells. The GO enrichment analysis results showed that differentially expressed genes were highly correlated with cellular biological processes such as cell cycle, apoptosis, intercellular signal transduction, and cell adhesion, as well as with cell components such as nucleus and cell membrane. KEGG pathway enrichment analysis showed that differentially expressed genes in drug-resistant cells were concentrated in MAPK signaling pathway, PI3K/Akt signaling pathway and cAMP signaling pathway.

In NCI-H460 human lung cancer cells, changes in the expression of genes such as ABCA9, CYR61, ABCC8, and CYP4F12 may enhance their resistance to MLN4924. This resistance may be related to the MAPK signaling pathway, PI3K Akt signaling pathway, and cAMP signaling pathway.

To investigate the effects of miR-15a-5p targeting heparanase2 (HPSE2) on the proliferation, migration, and invasion of cervical cancer cells.

Human cervical cancer cell lines (Hela and SIHA) were used as the research objects. miR-15a-5p mimic, miR-15a-5p inhibitor and HPSE2 plasmid were transfected into cells. The cell viability was detected by CCK-8, and the cell migration and invasion ability was detected by scratch test and Transwell assay, respectively. Western blot was used to detect the expression of HPSE2, and RT-qPCR was used to detect the levels of miR-15a-5p and HPSE2 mRNA in cells. Independent sample t-test was used for comparison between two groups, one-way analysis of variance was used for comparison between multiple groups, and LSD-t test was used for pairwise comparison between multiple groups.

Compared with human normal cervical epithelial cells (HcerEpic) , the levels of miR-15a-5p were increased in cervical cancer cells Hela, HeRC32 and SIHA [ (3.11 ± 0.32) , (1.51 ± 0.07) , (1.64 ± 0.09) vs (1.00 ± 0.05) ]. The mRNA level of HPSE2 were decreased [ (0.29 ± 0.04) , (0.45 ± 0.09) , (0.45 ± 0.10) vs (1.00 ± 0.05) ] (all P < 0.05) . Compared with the control, transfection of miR-15a-5p inhibitor inhibited the proliferation [72 h: (0.85 ± 0.08) vs (1.10 ± 0.12) , (0.76 ± 0.12) vs (1.04 ± 0.11) ], migration [ (12.67 ± 2.52) % vs (23.00 ± 3.00) %, (14.33 ± 2.52) % vs (24.67 ± 2.52) %] and invasive ability of cervical cancer cells Hela and SIHA [ (59.33 ± 11.24) vs (127.00 ± 12.49) , (65.67 ± 14.05) vs (136.00 ± 15.52) ]; Compared with the control, miR-15a-5p mimic further inhibited the expression of HPSE2 protein in cervical cancer cells Hela and SIHA [ (0.61 ± 0.02) vs (1.00 ± 0.03) , (0.34 ± 0.05) vs (1.00 ± 0.05) ]. Compared with miR-15a-5p mimic, HPSE2 overexpression increased the protein level of HPSE2 in cervical cancer cells Hela and SIHA [ (2.14 ± 0.13) vs (1.00 ± 0.12) , (1.71 ± 0.02) vs (1.00 ± 0.03) ]. Overexpression of HPSE2 reversed the effect of miR-15a-5p mimic and inhibit the proliferation [72 h: (0.83 ± 0.10) vs (1.44 ± 0.14) , (0.93 ± 0.06) vs (1.25 ± 0.10) ], migration [ (34.00 ± 5.57) % vs (43.67 ± 10.41) %, (33.00 ± 3.61) % vs (41.00 ± 8.00) %] and invasive ability of cervical cancer cells Hela and SIHA [ (158.00 ± 13.89) vs (260.66 ± 15.26) , (150.67 ± 23.54) vs (270.00 ± 12.77) ].

miR-15a-5p promotes the proliferation, migration and invasion of cervical cancer cells by regulating HPSE2.

To investigate the role of miR-126-3p/PIK3R1 axis proliferation, migration and invasion cells in ovarian cancer.

miR-126-3p mimic, miR-126-3p inhibitor and PIK3R1 plasmid were transfected into SKOV3 and A2780 ovarian cancer cells. Cell proliferation/migration was assessed by CCK-8, scratch and Transwell assays. Western blot and qPCR were used to detect the expression levels of PIK3R1 and miR-126-3p. A Dual-luciferase reporter assay was used to detect the target gene of miR-126-3p, PIK3R1. An independent samle t-test was used to compare between two groups, a one-way analysis of variance was used to compare among multiple groups and an LSD-t test was used for pairwise comparison among various groups.

Compared with the normal ovarian epithelial cell line IOSE80, the expression levels of miR-126-3p were up-regulated in ovarian cancer cell lines SKOV3, A2780, HO-8910 [ (2.30 ± 0.18) , (1.86 ± 0.11) , (1.26 ± 0.12) vs (1.00 ± 0.07) ] (all P < 0.05) ; Inhibition of miR-126-3p expression inhibited the proliferation [SKOV3 cells : 72 h (0.94 ± 0.13) vs (1.14 ± 0.08) , A2780 cells: 72 h (0.83 ± 0.09) vs (1.11 ± 0.12) ], migration [ (23.00 ± 6.08) % vs (37.67 ± 6.43) %, (25.67 ± 4.04) % vs (40.00 ± 6.58) %], invasion of SKOV3, A2780 cells[ (97.00 ± 17.35) vs (134.33 ± 13.32) , (97.00 ± 7.00) vs (127.00 ± 9.17) cells] (all P < 0.05) . And dual luciferase report experiment verified miR-126-3p targeted PIK3R1 gene in SKOV3 and A2780 cells. After overexpression of miR-126-3p, the expression of PIK3R1 protein was down-regulated in SKOV3 and A2780 cells [ (0.50 ± 0.08) vs (1.01 ± 0.04) , (0.54 ± 0.03) vs (1.00 ± 0.03) ] (all P < 0.001) . Compared with miR-126-3p mimic, miR-126-3p mimic+pPIK3R1 inhibited the proliferation [SKOV3 cells: 72 h (1.04 ± 0.14) vs (1.55 ± 0.12) , A2780 cells: 72 h (0.87 ± 0.09) vs (1.32 ± 0.11) ], migration ability[ (27.00 ± 2.00) %vs (34.00 ± 2.00) %, (24.67 ± 3.22) %vs (33.00 ± 4.00) %], invasion ability of SKOV3 and A2780 cells [ (60.67 ± 7.64) vs (135.00 ± 6.00) , (63.33 ± 10.02) vs (125.67 ± 9.87) cells], the protein expression of PIK3R1 was increased [ (1.92 ± 0.16) vs (1.00 ± 0.11) , (3.15 ± 0.06) vs (0.99 ± 0.12) ] (all P < 0.05) .

miR-126-3p promotes the proliferation, migration and invasion of ovarian cancer cells by targeting PIK3R1.

The aim of this study is to evaluate the changes of pupil diameter and dynamics in patients with different ametropia after implantable collamer lens (ICL)V4c without viscoelastic agent.

A Total of 53 patients (104 eyes) who underwent ICL V4c implantation surgery in the Department of Ophthalmology, Anzhen Hospital in Beijing, China from August 2019 to January 2021 were collected. Among them, there were 11 males (22 eyes) and 42 females (84 eyes) with the average age of (33.5±6.9) years old (ranged from 21 to 48) years old. According to the preoperative equivalent spherical refractive index of patients, they are divided into moderate to low myopia group and high myopia group. The patient's intraocular pressure, equivalent spherical lens, anterior chamber depth, central corneal thickness, and white to white diameter, the pupil diameter before and one month after ICL V4c implantation were performed. Age, intraocular pressure, pupil diameter, and changes in pupil motility and other quantitative data have undergone normality tests and conformed to a normal distribution, were expressed as ±s, and compared by independent sample t-tests for inter group, and paired t-tests for intra group. Quantitative data with equivalent spherical mirrors that did not follow a normal distribution were expressed as median (interquartile range), and compared by Mann Whitney U test for inter group, and Wilcoxon signed rank test for intra group. Gender was expressed in examples and percentages, and compared by χ² for inter group. The correlation analysis between preoperative and postoperative changes in pupil dynamics between two groups was performed using Pearson or Spearman correlation analysis.

Under bright light, the preoperative and postoperative pupil diameters of patients in moderate to low degree myopia group were (4.16±0.60) mm and (3.99±0.65) mm, respectively, with a statistical significance difference between them (t=2.338, P<0.05). Under dark light, the preoperative and postoperative pupil diameters were 5.76 (1.58) mm and 5.48 (1.13) mm, respectively, with no statistically significant difference (Z=1.873, P>0.05). The pupil diameter of moderate to low degree myopia group significantly decreased under postoperative bright light. The preoperative and postoperative pupil diameter of the high myopia group was 4.18 (1.00) mm and 4.17 (1.08) mm, respectively. The differencewas statistically significant between the two groups (Z=2.978, P<0.05). The pupil diameters of the highly myopic group before and after surgery under bright light were 4.18 (1.00) mm and 4.17 (1.08) mm, respectively, with no statistically significant difference (t=-1.004, P>0.05). Under dark light, the preoperative and postoperative pupil diameters were (5.81±0.84) mm and (5.86±0.89) mm, respectively. There was no statistically significant difference in preoperative pupil diameter between the two groups (Z=-1.510, -2.030; P>0.05). Under bright light, the difference in postoperative pupil diameter between the two groups was statistically significant (Z=-0.688, P<0.05). Under dark light, the postoperative pupil diameter was statistically significant (t=-0.02, P<0.05). The difference in pupil diameter dynamics before and after surgery was 1.29 (1.07) mm and (1.48±0.33) mm in the moderate to low degree myopia group, respectively, and there was no statistically significant difference between them (Z=-0.111, P>0.05). The difference in pupil dynamics before and after surgery in the high myopia group was (1.39±0.45) mm, and (1.59±0.53) mm, with a statistical significance difference (t=-3.265, P<0.05). The postoperative changes in pupil dynamics in the high myopia group were significantly larger than that of before surgery. The difference in pupil diameter between the two groups before and after surgery was not statistically significant (Z=-0.306, -0.721; P>0.05). Under bright light, the difference between the postoperative and preoperative pupil diameter in the medium to low degree myopia group was (-0.17±0.48) mm, and under dark light that was -0.09 (0.25) mm, both showing a decreasing trend, with no statistically significant difference (Z=0.000, P>0.05). Under bright light, the difference in postoperative and preoperative pupil diameter in the high myopia group was -0.17 (0.43) mm, and under dark that was (0.05±0.41) mm with statistically significant between them (Z=2.411, P<0.05). The high myopia group showed an increasing trend in postoperative pupil diameter under dark. Spearman correlation analysis showed a positive correlation between the preoperative and the postoperative pupil dynamics with statistical significance in low degree myopia group (r=0.587, P<0.05). Pearson correlation analysis showed a positive correlation between the preoperative and postoperative pupil dynamics, with a statistical significance difference between them (r=0.518, P<0.05).

The pupil diameter of the patients with moderate to low myopia and high myopia can be significantly reduced after ICL V4c implantation for one month without viscoviscoid-assisted implantation under bright light. In patients with high myopia, the pupil diameter changes more greatly under light and dark lighting after surgery, which is more likely to affect the visual quality.

The aim of this study is to investigate the clinical efficacy of epithelium-off versus transepithelial accelerated corneal collagen cross-linking (ACXL) in the treatment of advanced keratoconus.

Fifty-three keratoconus patients (61 eyes) who underwent ACXL were selected from August 2019 to October 2021 at laser Myopia Treatment Center of Xi′an First Hospital for retrospective study. Among them, there were 30 cases (32 eyes) male and 23 cases (29 eyes) female with the mean age of (24.3±6.9) years. Patients were divided into epithelium-off group (31 eyes) and transepithelial group (30 eyes) according to different ACXL operation types. The preoperative and postoperative at 3 months, 6 months, 12 months, 18 months, 24 months uncorrected visual acuity (UCVA), the best corrected visual acuity (BCVA), spherical equivalent (SE), the corneal astigmatism (CA), theanterior flat keratometry (K1), theanterior steep keratometry (K2), the maximal anterior keratometric power (Kmax), the posterior corneal curvature (PCC), and the thinnest corneal thickness (TCT) were performed and recorded the postoperative failure rate. UCVA, BCVA, TCT, K1, K2, Kmax, PCC, CA and SE were in accordance with normal distribution, and expressed as ±s and compared by independent sample t-test for intra groups before operation, multi-sample repeated measurement analysis of variance for inter-group, and repeated measurement analysis of variance for intra-group before and after treatment. The postoperative failure rate of the two cross-linking operations was expressed as eye number and percentage, and compared by Fisher′s exact test.

After surgery for 24 months, UVCA, BCVA, SE, CA, K1, K2, Kmax, PCC and TCT of patients in the epithelium-off group were (0.83±0.36) logarithmic minimum angle of resolution (logMAR), (0.20±0.15)logMAR, (-8.84±4.20)D, (-3.38±2.14)D, (44.85±1.74)D, (48.16±2.27)D, (49.86±10.51)D, (-6.89±0.48)D and (456.80±33.69)μm, respectively; those of patients in the transepithelial group were (1.01±0.46)logMAR, (0.33±0.66)logMAR, (-7.78±6.19)D, (-2.67±2.34)D, (46.63±3.40)D, (49.59±3.54)D, (55.92±7.69)D, (-7.15±0.55)D及(442.93±34.49)μm, respectively. There was no significant difference in the interaction of UVCA, BCVA, SE, CA, K1, K2, Kmax, PCC and TCT at different time point between the two groups (F_interaction=0.56, 0.80, 0.84, 0.32, 0.59, 1.70, 1.07, 0.31, 1.93; P>0.05). The difference in BCVA between the two groups was statistically significant (F_intra-group=4.27, P<0.05). There were significant differences in K2 at each time point in epithelium-off group (F=7.96, P<0.05). There were significant differences in Kmax at each time point in epithelium-off group (F_time=3.56, P<0.05). There were significant differences in TCT of epithelium-off group at each time point (F_time=7.72, P<0.05). After operation for one year, the failure rate of epithelium-off group and transepithelial group were 2 eyes (6.45%) and 6 eyes (20.00%), respectively, and there was no significant difference between them(P>0.05).

Long-term observation showed that epithelium-off CXL and transepithelial CXL are safe and effective in stabilizing keratoconus morphology and controlling keratoconus progression. In advanced keratoconus patients with the thinnest corneal thickness greater than 400 μm, epithelium-off CXL was superior to transepithelial CXL in controlling vision, corneal curvature and astigmatism.

The aim of this study is to analyze the correlation between baseline cytokine levels in the aqueous humor and the response to anti-vascular endothelial growth factor (VEGF) treatment in DME patients.

The patients with diabetic macular edema (DME) who received intravitreal injection of Aflibercept for 3 times in Shenyang Aier Excellence Eye Hospital from October 2021 to July 2023 were collected. Among them, there were 36 males (36 eyes) and 29 females (29 eyes)with an average age of (54.5±11.4) years (ranged from 29 to 79) years old. According to the decrease of central macular thickness (CMT) after three months of treatment, all patients were divided into response group and non-response group. Best corrected visual acuity (BCVA), intraocular pressure (IOP), CMT, and the aqueous cytokine levels of patients before and after treatment for 3 months were detected. BCVA, age, course of diabetes, IOP and other measurement data, after the normal test, if conformed to the normal distribution, were expressed as ±s, and compared by by independent sample t test for the inter group , and paired t test for the intra group. Quantitative data that did not follow a normal distribution were represented as median and interquartile (non interquartile range) and compared using Mann-Whitney U test. Gender was represented by cases and percentages, and compared using χ². Using multiple logistic regression analysis was to explore potential risk factors for non response to anti VEGF drugs. The correlation between different cytokines and the decrease in CMT was used Spearman correlation analysis.

The BCVA of the affected eye before and after treatment were (1.10±0.27) logarithm of the minimum angle of resolution (logMAR) and (0.61±0.30) logMAR, respectively. The CMT of the affected eye before and after treatment were (583.54±108.05)μm, and (419.95±80.01) μm, respectively . The differences were statistically significant (t=12.166, 11.447; P<0.05). The IOP of the affected eye before and after treatment were (15.98±2.68) mmHg (1 mmHg=0.133 kPa) and (15.88±2.48) mmHg, respectively, with no statistically significant difference (t=0.736, P>0.465). The baseline aqueous humor VEGF, intercellular adhesion molecule (ICAM), and interleukin (IL)-8 levels in the non-response group were 114.00(88.75, 156.85)pg/ml, 1891.30(1124.60, 2673.00)pg/ml and 57.70(49.40, 76.15)pg/ml, respectively, while those in the responsive group were 190.40(147.50, 333.70)pg/ml, 6821.80(4387.15, 9079.75)pg/ml and 26.50(19.70, 40.90)pg/ml, with statistical significance between them (Z=3.612, 6.291, 5.700; P<0.05). The levels of basic fibroblast growth factor (bFGF), IL-6, and vascular cell adhesion molecule (VCAM) in aqueous humor of the non-response group were 8.90 (4.80, 22.05)pg/ml, 61.90 (19.35, 134.60)pg/ml, and 2528.60 (919.65, 3353.30)pg/ml, respectively, while those of the responsive group were 13.80 (4.20, 31.95)pg/ml, 76.00 (31.70, 235.9)pg/ml, and 2480.60 (1086.55, 3656.00)pg/ml, with no statistically significant differences (Z=0.585, 1.596, 0.525; P>0.05). The age, course of disease, glycated hemoglobin, baseline visual acuity, baseline IOP, baseline CMT, as well as aqueous humor VEGF, bFGF, IL-6, VCAM, IL-8, and ICAM were used for multivariate logistic regression analysis to explore impact factors for anti VEGF treatment response, but no independent risk factors were found (β=-0.466, -2.012, 6.723, -8.634, 3.664, -0.009, 0.049, -0.109, -0.032, 0.007, -1.068, 0.015; P>0.05). According to Spearman correlation analysis, the levels of VEGF and ICAM were positively correlated with the decrease in CMT, and there was statistical significance (r=0.262, 0.750, P<0.05). The level of IL-8 was negatively correlated with the decrease in CMT with a statistical significance (r=-0.628, P<0.05). The levels of bFGF, IL-6, and VCAM were not correlated with CMT decrease without a statistically significance (r=0.127, 0.098, -0.241; P>0.05).

The levels of VEGF, ICAM-1, and IL-8 in aqueous humor are correlated with treatment response, which can be used to predict the therapeutic effect of anti VEGF in DME patients.

To investigate the effect of electroacupuncture on weight loss in obese patients pre-laparoscopic sleeve gastrectomy(LSG).

Clinical data of 50 obese patients who underwent LSG in Shanghai Ninth People's Hospital from August 2018 to May 2021 were included and analyzed. There were 25 cases received electroacupuncture combined with LSG (electroacupuncture combined with sleeve stomach group) and 25 cases received LSG (sleeve gastrectomy group). The clinical data of preoperative weight loss, length of LSG, hospital stay, complication rate and postoperative excess weight loss percentage were compared between the two groups.

The preoperative weight loss effect of electroacupuncture combined with sleeve stomach group was significantly better than sleeve stomach group. The operation time, total hospital stay, postoperative hospital stay and postoperative complication rate of electroacupuncture combined with sleeve stomach group were significantly lower than those of sleeve gastrectomy group. The %EWL of electroacupuncture combined with sleeve stomach group was significantly better than that of sleeve gastrectomy group at 6 and 12 months after LSG.

Electroacupuncture can significantly reduce the preoperative weight of obese patients before LSG, shorten the operation time and improve the postoperative weight loss effect. However, further RCTs of multi-center and large sample are still needed for verification.